Anticorps Monoclonal anti-PDCD4

• Phare
• Validé par KD/KO

PDCD4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F1A11

N° de cat : 66100-1-PBS

Synonymes

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

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Galerie de données de validation

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  WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

  WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66100-1-Ig (same clone as 66100-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66100-1-PBS cible PDCD4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDCD4 Protéine recombinante Ag19239
• Nom complet: programmed cell death 4 (neoplastic transformation inhibitor)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC026104
• Symbole du gène: PDCD4
• Identification du gène (NCBI): 27250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

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