Anticorps Recombinant de lapin anti-PDCD4

PDCD4 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241463E3

N° de cat : 84162-3-RR

Synonymes

Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug

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Galerie de données de validation

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  WB analysis using 84162-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84162-3-RR (PDCD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84162-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDCD4 Recombinant antibody (84162-3-RR, Clone:241463E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84162-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84162-3-RR against Human PDCD4 were performed. The affinity constant is 1.54 nM.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HEK-293, cellules HeLa, cellules HUVEC, cellules MCF-7
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84162-3-RR cible PDCD4 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDCD4 Protéine recombinante Ag3288
• Nom complet: programmed cell death 4 (neoplastic transformation inhibitor)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 54-64 kDa
• Numéro d’acquisition GenBank: BC026104
• Symbole du gène: PDCD4
• Identification du gène (NCBI): 27250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.

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