Anticorps Polyclonal de lapin anti-PDCD5

• Phare
• Validé par KD/KO

PDCD5 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 12456-1-AP

Synonymes

PDCD5, programmed cell death 5, Protein TFAR19, TFAR19

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Galerie de données de validation

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  WB analysis of human heart using 12456-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12456-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12456-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12456-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules A549, cellules PC-3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

12456-1-AP cible PDCD5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDCD5 Protéine recombinante Ag3122
• Nom complet: programmed cell death 5
• Masse moléculaire calculée: 125 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC015519
• Symbole du gène: PDCD5
• Identification du gène (NCBI): 9141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDCD5, also called TFAR19 (TF1 cell apoptosis-related gene 19), was first identified as a gene up-regulated in TF-1 cells under-going apoptosis (PMID: 9920759). PDCD5 can promote pro-grammed cell death in different cell types in response to various stimuli and also enhance TAJ/TROY-induced paraptosis-like cell death (PMID: 15020679). During apoptosis, PDCD5 is rapidly upregulated and translocates from the cytoplasm to nucleus (PMID: 11741587). PDCD5 is a positive regulator of Tip60 and also has a potential ability to interact with p53 (PMID: 22914926, PMID: 19308289). Recent studies have also revealed that PDCD5 may be a suppressor gene and expressed at lower levels in many cancers, including hepatocellular carcinoma, breast cancer, gastric cancer, cervical cancer, lung cancer, astrocytic gliomas, and in leukemia.

Publications

Species	Application	Title
	IHC, WB	Nat Commun; Club cell-specific role of programmed cell death 5 in pulmonary fibrosis.; Authors - Soo-Yeon Park
	WB	Nat Commun; Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.; Authors - Hyo-Kyoung Choi ; KD Validated
human	WB	J Nanobiotechnology; Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.; Authors - Lin Lin
human	WB	J Cell Physiol; Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.; Authors - Dong Lin
human	WB	Sci Rep; Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.; Authors - Soo-Yeon Park
human	WB, IF	Cancer Lett; DNAJB1 destabilizes PDCD5 to suppress p53-mediated apoptosis.; Authors - Xiandan Cui