Anticorps Recombinant de lapin anti-PDCD6/ALG2

PDCD6/ALG2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250087B4

N° de cat : 85792-5-RR

Synonymes

ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog

---

Galerie de données de validation

• 

  WB analysis using 85792-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 85792-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85792-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85792-5-RR against Human PDCD6/ALG2 were performed. The affinity constant is 5.93 nM.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85792-5-RR cible PDCD6/ALG2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDCD6/ALG2 Protéine recombinante Ag2949
• Nom complet: programmed cell death 6
• Masse moléculaire calculée: 191 aa, 22 kDa
• Poids moléculaire observé: 15-22 kDa
• Numéro d’acquisition GenBank: BC012384
• Symbole du gène: PDCD6
• Identification du gène (NCBI): 10016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death protein 6 (PDCD6) is also named as ALG2. PDCD6 is an evolutionarily conserved Ca2+-binding protein. PDCD6 is required for coat protein complex II-dependent endoplasmic reticulum-to-Golgi apparatus vesicular transport in the cytoplasm (PMID: 39934130). PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. The nuclear PDCD6 regulates apoptosis and alternative splicing. And cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex-dependent membrane budding. (PMID: 38436472). PDCD6 also acts as a regulator of cytosolic DNA-induced immune responses by modulating STING transport (PMID: 34787301).

{{ptg:RelatedPrimaryAntibodies}}