Anticorps Polyclonal de lapin anti-PDE1A

• Phare
• Validé par KD/KO

PDE1A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12442-2-AP

Synonymes

61 kDa Cam PDE, Cam PDE 1A, hCam 1, HCAM1, HSPDE1A, PDE1A

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Galerie de données de validation

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  WB analysis using 12442-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12442-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12442-2-AP

  IP result of anti-PDE1A(IP:12442-2-AP, 4ug; Detection:12442-2-AP 1:1000) with mouse brain tissue lysate 2080 ug.

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 25x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

12442-2-AP cible PDE1A dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDE1A Protéine recombinante Ag3157
• Nom complet: phosphodiesterase 1A, calmodulin-dependent
• Masse moléculaire calculée: 545 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC022480
• Symbole du gène: PDE1A
• Identification du gène (NCBI): 5136
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).

Publications

Species	Application	Title
		Biol Reprod; Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis.; Authors - Jeremy R Egbert
mouse	WB	Biol Reprod; Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Generation and phenotypic characterization of Pde1a mutant mice.; Authors - Xiaofang Wang ; KO Validated
		Cell Rep; Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development.; Authors - Ashley G Anderson
human	IHC	Front Genet; Transcriptome profiling of intact bowel wall reveals that PDE1A and SEMA3D are possible markers with roles in enteric smooth muscle apoptosis, proliferative disorders, and dysautonomia in Crohn's disease; Authors - Yun Yang