Anticorps Monoclonal anti-PDE4D

PDE4D Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1C4B9

N° de cat : 67062-1-Ig

Synonymes

1C4B9, 3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53



Applications testées

Résultats positifs en WBcellules A549, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
Résultats positifs en IF/ICCcellules A431,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

67062-1-Ig cible PDE4D dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PDE4D Protéine recombinante Ag8302
Nom complet phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
Masse moléculaire calculée 809 aa, 91 kDa
Poids moléculaire observé 91-110 kDa, 66-84 kDa
Numéro d’acquisition GenBankBC036319
Symbole du gène PDE4D
Identification du gène (NCBI) 5144
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).

Protocole

Product Specific Protocols
WB protocol for PDE4D antibody 67062-1-IgDownload protocol
IF protocol for PDE4D antibody 67062-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Angie (Verified Customer) (06-06-2023)

Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 67062-1-Ig (mouse anti-PDE4D antibody) at dilution of 1:10 000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10 000
  • Cell Tissue Type: Human lung cancer cell A549
PDE4D Antibody Western Blot validation (1:10 000 dilution) in Human lung cancer cell A549 (Cat no:67062-1-Ig)
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