Anticorps Monoclonal anti-PDE4D
PDE4D Monoclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, rat, souris
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
1C4B9
N° de cat : 67062-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A549, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris |
Résultats positifs en IF/ICC | cellules A431, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:3000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 1 publications below |
Informations sur le produit
67062-1-Ig cible PDE4D dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
Réactivité | Humain, porc, rat, souris |
Réactivité citée | Humain |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | PDE4D Protéine recombinante Ag8302 |
Nom complet | phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) |
Masse moléculaire calculée | 809 aa, 91 kDa |
Poids moléculaire observé | 91-110 kDa, 66-84 kDa |
Numéro d’acquisition GenBank | BC036319 |
Symbole du gène | PDE4D |
Identification du gène (NCBI) | 5144 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).
Protocole
Product Specific Protocols | |
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WB protocol for PDE4D antibody 67062-1-Ig | Download protocol |
IF protocol for PDE4D antibody 67062-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Angie (Verified Customer) (06-06-2023) | Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 67062-1-Ig (mouse anti-PDE4D antibody) at dilution of 1:10 000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.
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