Anticorps Monoclonal anti-PDE4D

PDE4D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C4B9

N° de cat : 67062-1-PBS

Synonymes

1C4B9, 3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53

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Galerie de données de validation

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  WB analysis using 67062-1-Ig (same clone as 67062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67062-1-Ig (same clone as 67062-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67062-1-Ig (same clone as 67062-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67062-1-Ig (same clone as 67062-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDE4D antibody (67062-1-Ig, Clone: 1C4B9 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67062-1-PBS cible PDE4D dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDE4D Protéine recombinante Ag8302
• Nom complet: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Masse moléculaire calculée: 809 aa, 91 kDa
• Poids moléculaire observé: 91-110 kDa, 66-84 kDa
• Numéro d’acquisition GenBank: BC036319
• Symbole du gène: PDE4D
• Identification du gène (NCBI): 5144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).

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