Anticorps Recombinant de lapin anti-PDE4D

PDE4D Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251427D3

N° de cat : 86745-1-RR

Synonymes

3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53, PDE43

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Galerie de données de validation

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  WB analysis of mouse pancreas using 86745-1-RR

  Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86745-1-RR

  Rat brain tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 86745-1-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86745-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86745-1-RR against Human PDE4D were performed. The affinity constant is 3.51 nM.

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, cellules K-562, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86745-1-RR cible PDE4D dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDE4D Protéine recombinante Ag3588
• Nom complet: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Masse moléculaire calculée: 809 aa, 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC036319
• Symbole du gène: PDE4D
• Identification du gène (NCBI): 5144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE4D (cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43, and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms, and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer's disease(PMID:17353396).

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