Anticorps Monoclonal anti-PDE6D

PDE6D Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1H4B6

N° de cat : 67565-1-PBS

Synonymes

1H4B6, PDED

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Galerie de données de validation

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  WB analysis of Jurkat using 67565-1-Ig (same clone as 67565-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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  WB analysis using 67565-1-Ig (same clone as 67565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67565-1-Ig (same clone as 67565-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat retina using 67565-1-Ig (same clone as 67565-1-PBS)

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67565-1-Ig (same clone as 67565-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67565-1-Ig (same clone as 67565-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67565-1-Ig (same clone as 67565-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67565-1-Ig (same clone as 67565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67565-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67565-1-PBS cible PDE6D dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDE6D Protéine recombinante Ag29563
• Nom complet: phosphodiesterase 6D, cGMP-specific, rod, delta
• Masse moléculaire calculée: 17 KDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: NM_002601
• Symbole du gène: PDE6D
• Identification du gène (NCBI): 5147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

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