Anticorps Polyclonal de lapin anti-PDE8A

PDE8A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13956-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 13956-1-AP (same clone as 13956-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 13956-1-AP (same clone as 13956-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13956-1-PBS cible PDE8A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDE8A Protéine recombinante Ag5069
• Nom complet: phosphodiesterase 8A
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 93 kDa, 88 kDa
• Numéro d’acquisition GenBank: BC060762
• Symbole du gène: PDE8A
• Identification du gène (NCBI): 5151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).

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