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Anticorps Recombinant de lapin anti-PDGF-BB

PDGF-BB Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

230486A7

N° de cat : 83214-5-PBS

Synonymes

PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using PDGF-BB Recombinant antibody (83214-5-RR)

    WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PDGF-BB Recombinant antibody (83214-5-RR)

    WB analysis of A549 using 83214-5-RR (same clone as 83214-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83214-5-RR

    Affinity and Kinetic Characterization of 83214-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is 16.9 nM.

Informations sur le produit

83214-5-PBS cible PDGF-BB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène PDGF-BB Protéine recombinante Ag25986
Nom complet platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
Masse moléculaire calculée 241 aa, 27 kDa
Poids moléculaire observé27 kDa
Numéro d’acquisition GenBankBC029822
Symbole du gène PDGFB
Identification du gène (NCBI) 5155
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

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