Anticorps Polyclonal de lapin anti-PDGFR beta/CD140b

• Phare
• Validé par KD/KO

PDGFR beta/CD140b Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 13449-1-PBS

Synonymes

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Galerie de données de validation

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  WB analysis using 13449-1-AP (same clone as 13449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 13449-1-AP (same clone as 13449-1-PBS)

  IP result of anti-PDGFR beta (IP:13449-1-AP, 4ug; Detection:13449-1-AP 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13449-1-PBS cible PDGFR beta/CD140b dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDGFR beta/CD140b Protéine recombinante Ag4285
• Nom complet: platelet-derived growth factor receptor, beta polypeptide
• Masse moléculaire calculée: 1106 aa, 124 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC032224
• Symbole du gène: PDGFR beta
• Identification du gène (NCBI): 5159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

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