Anticorps Recombinant de lapin anti-PDGFR beta/CD140b

PDGFR beta/CD140b Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242711A9

N° de cat : 98405-2-RR

Synonymes

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Galerie de données de validation

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  FC experiment of MG-63 using 98405-2-RR

  1x10^6 MG-63 cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells or PDGFR beta/CD140b transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells (left) or PDGFR beta/CD140b transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human PDGFRB Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: MG-63 cells, Transfected HEK-293T cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98405-2-RR cible PDGFR beta/CD140b dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDGFR beta/CD140b Protéine recombinante Eg3715
• Nom complet: platelet-derived growth factor receptor, beta polypeptide
• Masse moléculaire calculée: 1106 aa, 124 kDa
• Numéro d’acquisition GenBank: BC032224
• Symbole du gène: PDGFR beta
• Identification du gène (NCBI): 5159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

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