Anticorps Recombinant de lapin anti-PDGFR alpha/CD140a

PDGFR alpha/CD140a Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241658H4

N° de cat : 98141-1-PBS

Synonymes

PDGFRA, 241658H4, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A

---

Galerie de données de validation

• 

  Sandwich ELISA standard curve of MP01256-4

  Sandwich ELISA standard curve of MP01256-4, Human PDGFR alpha/CD140a Recombinant Matched Antibody Pair - PBS only. 84383-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00003. 98141-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Sample test of MP01256-4

  Serum of six individual healthy human donors was measured. The PDGFR alpha/CD140a concentration of detected samples was determined to be 2,557.7 pg/mL with a range of 1,472.3-4,545.6 pg/mL.

• 

  Sample test of MP01256-4

  The mean PDGFR alpha/CD140a concentration was determined to be 2.6 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load and 7.0 ng/mL in human placenta tissue extract based on a 1.7 mg/mL extract load.

• 

  FC experiment of MG-63 using 98141-1-RR (same clone as 98141-1-PBS)

  1x10^6 MG-63 cells (left) or A-172 cells (right) were surface stained with 0.25 ug Anti-Human PDGFR alpha/CD140a Rabbit Recombinant Antibody (98141-1-RR, Clone: 241658H4) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98141-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98141-1-RR against Human PDGFR alpha/CD140a were performed. The affinity constant is 2.26 nM.

---

Informations sur le produit

98141-1-PBS cible PDGFR alpha/CD140a dans les applications de FC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: platelet-derived growth factor receptor, alpha polypeptide
• Masse moléculaire calculée: 123 kDa
• Numéro d’acquisition GenBank: BC015186
• Symbole du gène: PDGFR alpha
• Identification du gène (NCBI): 5156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

{{ptg:RelatedPrimaryAntibodies}}