Anticorps Polyclonal de lapin anti-PDGFR beta/CD140b

• Phare
• Validé par KD/KO

PDGFR beta/CD140b Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(49)

Avis (1)

N° de cat : 13449-1-AP

Synonymes

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Galerie de données de validation

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  WB analysis using 13449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13449-1-AP

  IP result of anti-PDGFR beta (IP:13449-1-AP, 4ug; Detection:13449-1-AP 1:5000) with mouse lung tissue lysate 1120 ug.

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  IHC staining of human placenta using 13449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris,
• Résultats positifs en IP: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

13449-1-AP cible PDGFR beta/CD140b dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDGFR beta/CD140b Protéine recombinante Ag4285
• Nom complet: platelet-derived growth factor receptor, beta polypeptide
• Masse moléculaire calculée: 1106 aa, 124 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC032224
• Symbole du gène: PDGFR beta
• Identification du gène (NCBI): 5159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

Publications

Species	Application	Title
rat	WB	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv ; KD Validated
human		Cell Rep; Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells; Authors - Hiraku Tsujimoto
mouse	WB	J Mol Cell Biol; BMP4 mediates the interplay between adipogenesis and angiogenesis during expansion of subcutaneous white adipose tissue.; Authors - Yan Tang
		Br J Cancer; Inhibition of PFKFB3 in HER2-positive gastric cancer improves sensitivity to trastuzumab by inducing tumour vessel normalisation.; Authors - Xingxing Yao
rat		Neurotherapeutics; A switch in the pathway of TRPC3-mediated calcium influx into brain pericytes contributes to capillary spasms after subarachnoid hemorrhage; Authors - Yuncong Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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