Anticorps Monoclonal anti-PDH E1 Alpha

• Phare
• Validé par KD/KO

PDH E1 Alpha Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

2B3C10

N° de cat : 66119-1-Ig

Synonymes

PDH E1 Alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit

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Galerie de données de validation

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  WB analysis of HEK293 cells using 66119-1-Ig

  WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells.

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  WB analysis of HEK-293 using 66119-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66119-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66119-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 66119-1-Ig

  IP Result of anti-PDH E1α (IP:66119-1-Ig, 3ug; Detection:66119-1-Ig 1:10000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human heart using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens).

• 

  IHC staining of human liver using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 66119-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66119-1-Ig (PDH E1α antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IgG.

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  FC experiment of HepG2 using 66119-1-Ig

  1X10^6 HepG2 cells were stained with .2ug PDH E1α antibody (66119-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66119-1-Ig cible PDH E1 Alpha dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDH E1 Alpha Protéine recombinante Ag12556
• Nom complet: pyruvate dehydrogenase (lipoamide) alpha 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC002406
• Symbole du gène: PDHA1
• Identification du gène (NCBI): 5160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publications

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
		J Cell Biol; PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy.; Authors - Kenneth R Pryde
human	WB	Cell Rep; A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.; Authors - Kenneth Robert Pryde
human	WB	Toxicol Appl Pharmacol; Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.; Authors - Jin-Long Pang
human	WB	Immunobiology; The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus.; Authors - Fang Wang
human	WB,IF	Nat Metab; MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.; Authors - Yajuan Zhang ; KD Validated