Anticorps Monoclonal anti-PDH E1 Alpha

• Phare
• Validé par KD/KO

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

CloneNo.

2B3C10

N° de cat : 66119-1-Ig

Synonymes

PDH E1α, PDHA1, 2B3C10, EC:1.2.4.1, PDHA

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Galerie de données de validation

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  WB analysis of HEK293 cells using 66119-1-Ig

  WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells.

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  WB analysis using 66119-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 66119-1-Ig

  IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 4ug; Detection:66119-1-Ig 1:10000) with K-562 cells lysate 1820 ug.

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66119-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66119-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDH E1 Alpha antibody (66119-1-Ig, Clone: 2B3C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), PDH E1 Alpha antibody (18068-1-AP, red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

66119-1-Ig cible PDH E1 Alpha dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDH E1 Alpha Protéine recombinante Ag12556
• Nom complet: pyruvate dehydrogenase (lipoamide) alpha 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 39-48 kDa
• Numéro d’acquisition GenBank: BC002406
• Symbole du gène: PDHA1
• Identification du gène (NCBI): 5160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publications

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
		J Cell Biol; PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy.; Authors - Kenneth R Pryde
human	WB	Cell Rep; A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.; Authors - Kenneth Robert Pryde
	WB	Oxid Med Cell Longev; NOX4-Derived ROS Mediates TGF- β 1-Induced Metabolic Reprogramming during Epithelial-Mesenchymal Transition through the PI3K/AKT/HIF-1 α Pathway in Glioblastoma; Authors - Xiangsheng Su
human	WB	Toxicol Appl Pharmacol; Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.; Authors - Jin-Long Pang
human	WB	Immunobiology; The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus.; Authors - Fang Wang

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