Anticorps Polyclonal de lapin anti-PDHX

• Phare
• Validé par KD/KO

PDHX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10951-1-PBS

Synonymes

PDX1, pyruvate dehydrogenase complex, component X, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3 binding protein

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Galerie de données de validation

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  WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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  WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 10951-1-AP (same clone as 10951-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

  IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10951-1-AP (same clone as 10951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10951-1-PBS cible PDHX dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDHX Protéine recombinante Ag1389
• Nom complet: pyruvate dehydrogenase complex, component X
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC010389
• Symbole du gène: PDHX
• Identification du gène (NCBI): 8050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

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