Anticorps Monoclonal anti-PDI

• Phare
• Validé par KD/KO

PDI Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E6A11

N° de cat : 66422-1-PBS

Synonymes

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

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Galerie de données de validation

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  WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66422-1-Ig (same clone as 66422-1-PBS)

  WB result of PDI antibody (66422-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66422-1-Ig (same clone as 66422-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50798-2

  Cytometric bead array standard curve of MP50798-2, PDI Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66422-1-PBS. Detection antibody: 66422-3-PBS. Standard:Ag1747. Range: 0.098-1.563 ng/mL.

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Informations sur le produit

66422-1-PBS cible PDI dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDI Protéine recombinante Ag1747
• Nom complet: prolyl 4-hydroxylase, beta polypeptide
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC014504
• Symbole du gène: PDI
• Identification du gène (NCBI): 5034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

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