Anticorps Recombinant de lapin anti-PDIA2

PDIA2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242294E10

N° de cat : 84765-4-PBS

Synonymes

242294E10, PDA2, PDI, PDIP, PDIR

---

Galerie de données de validation

• 

  WB analysis using 84765-4-RR (same clone as 84765-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84765-4-RR (PDIA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84765-4-RR (same clone as 84765-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84765-4-RR (PDIA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84765-4-RR (same clone as 84765-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84765-4-RR (PDIA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84765-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84765-4-RR against Human PDIA2 were performed. The affinity constant is 24.0 pM.

---

Informations sur le produit

84765-4-PBS cible PDIA2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDIA2 Protéine recombinante Ag35301
• Nom complet: protein disulfide isomerase family A, member 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: NM_006849.2
• Symbole du gène: PDIA2
• Identification du gène (NCBI): 64714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)

{{ptg:RelatedPrimaryAntibodies}}