Anticorps Recombinant de lapin anti-PDIA4

PDIA4 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250697E10

N° de cat : 86239-1-PBS

Synonymes

ERp72, EC:5.3.4.1, ERp 72, ERp70, ERp-72

---

Galerie de données de validation

• 

  WB analysis using 86239-1-RR (same clone as 86239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86239-1-RR (PDIA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86239-1-RR (same clone as 86239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDIA4 antibody (86239-1-RR, Clone: 250697E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

86239-1-PBS cible PDIA4 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDIA4 Protéine recombinante Eg3011
• Nom complet: protein disulfide isomerase family A, member 4
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_004911.5
• Symbole du gène: ERp72
• Identification du gène (NCBI): 9601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

{{ptg:RelatedPrimaryAntibodies}}