Anticorps Polyclonal de lapin anti-PDIR

• Phare
• Validé par KD/KO

PDIR Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15545-1-AP

Synonymes

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

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Galerie de données de validation

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  WB analysis of mouse liver using 15545-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15545-1-AP (PDIR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15545-1-AP

  IP result of anti-PDIR (IP:15545-1-AP, 4ug; Detection:15545-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

• 

  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique humain, tissu ovarien humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15545-1-AP cible PDIR dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDIR Protéine recombinante Ag7901
• Nom complet: protein disulfide isomerase family A, member 5
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC001625
• Symbole du gène: PDIR
• Identification du gène (NCBI): 10954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER.; Authors - Richard Zimmermann
human	WB,IHC	Oncotarget; Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.; Authors - Soma Samanta
human	WB	Viruses; PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection; Authors - Mengwei Xu ; KD Validated
human	WB	FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
mouse	WB	JCI Insight; Protein disulfide isomerase is essential for spermatogenesis in mice; Authors - Yaqiong Zhang
human	WB,IF	Int J Biol Sci; RUNX1-PDIA5 Axis Promotes Malignant Progression of Glioblastoma by Regulating CCAR1 Protein Expression; Authors - Qiankun Ji ; KD Validated

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