Anticorps Monoclonal anti-PDIA6

PDIA6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G2D4

N° de cat : 66669-1-PBS

Synonymes

EC:5.3.4.1, ER protein 5, ERp5, P5, Protein disulfide isomerase A6

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Galerie de données de validation

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  WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66669-1-PBS cible PDIA6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDIA6 Protéine recombinante Ag27608
• Nom complet: protein disulfide isomerase family A, member 6
• Masse moléculaire calculée: 48 kDa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC001312
• Symbole du gène: PDIA6
• Identification du gène (NCBI): 10130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.

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