Anticorps Polyclonal de lapin anti-PDK2

• Phare
• Validé par KD/KO

PDK2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15647-1-AP

Synonymes

PDHK2, PDK2

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Galerie de données de validation

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  WB analysis of human heart using 15647-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15647-1-AP (PDK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15647-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15647-1-AP (PDK2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 15647-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 15647-1-AP (PDK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15647-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15647-1-AP (PDK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15647-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15647-1-AP (PDK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 15647-1-AP

  IP Result of anti-PDK2 (IP:15647-1-AP, 4ug; Detection:15647-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug.

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  IHC staining of mouse brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15647-1-AP (PDK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15647-1-AP (PDK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15647-1-AP (PDK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15647-1-AP (PDK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15647-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15647-1-AP (PDK2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HeLa, cellules NCI-H1299, tissu cardiaque de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu placentaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 5 publications below

Informations sur le produit

15647-1-AP cible PDK2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDK2 Protéine recombinante Ag8183
• Nom complet: pyruvate dehydrogenase kinase, isozyme 2
• Masse moléculaire calculée: 407 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC005811
• Symbole du gène: PDK2
• Identification du gène (NCBI): 5164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDK2([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial) is also named as PDHK2 and belongs to the PDK/BCKDK protein kinase family. PDK2 plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDK2 is also involved in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.; Authors - Biying Zhu
human	IHC	Hepatology; Retinoic acid-related orphan receptor alpha reprograms glucose metabolism in glutamine-deficient hepatoma cells.; Authors - Jun-Kyu Byun
human	IHC	Oncogene; Dichloroacetate restores colorectal cancer chemosensitivity through the p53/miR-149-3p/PDK2-mediated glucose metabolic pathway.; Authors - Yu Liang
human	IHC	Mol Ther Oncolytics; ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.; Authors - Hengqiang Zhao
human	WB	Sci Rep; Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.; Authors - Pengying Li
human	IHC	Am J Physiol Lung Cell Mol Physiol; Contribution of Fatty Acid Oxidation to the Pathogenesis of Pulmonary Hypertension.; Authors - Michael Hyunjean Lee