Anticorps Polyclonal de lapin anti-PDK3

PDK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12215-1-AP

Synonymes

PDK3

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Galerie de données de validation

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  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12215-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12215-1-AP

  IP result of anti-PDK3 (IP:12215-1-AP, 4ug; Detection:12215-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

12215-1-AP cible PDK3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDK3 Protéine recombinante Ag2879
• Nom complet: pyruvate dehydrogenase kinase, isozyme 3
• Masse moléculaire calculée: 406 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC015948
• Symbole du gène: PDK3
• Identification du gène (NCBI): 5165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).

Publications

Species	Application	Title
human	WB	Cancer Res; IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism.; Authors - Jose L Izquierdo-Garcia
human	IHC	Front Oncol; Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma; Authors - Yu-Hsuan Kuo
human	WB,IHC	Mol Cell Biochem; Lycorine hydrochloride interferes with energy metabolism to inhibit chemoresistant glioblastoma multiforme cell growth through suppressing PDK3; Authors - Qingsheng Dong
mouse	WB,IHC	Environ Pollut; Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse; Authors - Yan-Zhu Chen
human	WB	Nat Commun; NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice; Authors - Jie Wang
mouse	WB	Commun Biol; Therapeutic potential of microglial SMEK1 in regulating H3K9 lactylation in cerebral ischemia-reperfusion; Authors - Wei-Yue Si

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