Anticorps Polyclonal de lapin anti-PDK4

• Phare
• Validé par KD/KO

PDK4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 12949-1-PBS

Synonymes

PDHK4, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

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Galerie de données de validation

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  WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 12949-1-AP (same clone as 12949-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12949-1-AP (same clone as 12949-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDK4 antibody (12949-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12949-1-PBS cible PDK4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDK4 Protéine recombinante Ag3629
• Nom complet: pyruvate dehydrogenase kinase, isozyme 4
• Masse moléculaire calculée: 411 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC040239
• Symbole du gène: PDK4
• Identification du gène (NCBI): 5166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

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