Anticorps Recombinant de lapin anti-PDK4

PDK4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240551F1

N° de cat : 83583-3-PBS

Synonymes

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00556-3

  Sandwich ELISA standard curve of MP00556-3, Human PDK4 Recombinant Matched Antibody Pair - PBS only. 83583-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3629. 83583-1-PBS was HRP conjugated as the detection antibody. Range: 0.47-30 ng/mL

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  Sample test of MP00556-3

  Dissect the rat heart tissue and wash briefly with chilled 1X PBS to remove any blood if necessary, cut the tissue into smaller pieces whilst keeping it on ice. Transfer the tissue to a homogenizer and add Extraction Reagent with protease inhibitor. In general, add 500 μL Extraction Reagent for approximately every 10 mg of tissue. Homogenize thoroughly and keep the sample on ice for 30 min. Sonicate the sample and centrifuge at 10,000 x g, then transfer the supernatant to assay. PDK4 in rat heart tissue mesured 38.41 ng/ml total protein.

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Informations sur le produit

83583-3-PBS cible PDK4 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDK4 Protéine recombinante Ag3629
• Nom complet: pyruvate dehydrogenase kinase, isozyme 4
• Masse moléculaire calculée: 411 aa, 46 kDa
• Numéro d’acquisition GenBank: BC040239
• Symbole du gène: PDK4
• Identification du gène (NCBI): 5166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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