Anticorps Recombinant de lapin anti-PDLIM4

PDLIM4 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241401B3

N° de cat : 84117-1-RR

Synonymes

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3

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Galerie de données de validation

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  WB analysis using 84117-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84117-1-RR (PDLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 84117-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 84117-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84117-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84117-1-RR against Human PDLIM4 were performed. The affinity constant is 2.43 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules K-562, cellules PC-3
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84117-1-RR cible PDLIM4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDLIM4 Protéine recombinante Ag13424
• Nom complet: PDZ and LIM domain 4
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC016765
• Symbole du gène: PDLIM4
• Identification du gène (NCBI): 8572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).

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