Anticorps Polyclonal de lapin anti-PDLIM7

• Phare
• Validé par KD/KO

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 17080-1-AP

Synonymes

ENIGMA, LIM mineralization protein, LMP1

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Galerie de données de validation

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  WB analysis of HeLa using 17080-1-AP

  WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

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  WB analysis using 17080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17080-1-AP

  IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

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  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17080-1-AP cible PDLIM7 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDLIM7 Protéine recombinante Ag1334
• Nom complet: PDZ and LIM domain 7 (enigma)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC014521
• Symbole du gène: PDLIM7
• Identification du gène (NCBI): 9260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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