Anticorps Recombinant de lapin anti-PDLIM7

PDLIM7 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250775F12

N° de cat : 86283-1-RR

Synonymes

ENIGMA, LIM mineralization protein, LMP1

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Galerie de données de validation

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  WB analysis using 86283-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86283-1-RR (PDLIM7,LMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of SKOV-3 using 86283-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDLIM7 antibody (86283-1-RR, Clone: 250775F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86283-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86283-1-RR against Human PDLIM7 were performed. The affinity constant is 8.36 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules SKOV-3
• Résultats positifs en IF/ICC: cellules SKOV-3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86283-1-RR cible PDLIM7 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDLIM7 Protéine recombinante Ag4614
• Nom complet: PDZ and LIM domain 7 (enigma)
• Masse moléculaire calculée: 55/60 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC001093
• Symbole du gène: PDLIM7
• Identification du gène (NCBI): 9260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

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