Anticorps Polyclonal de lapin anti-PDPK1

• Phare
• Validé par KD/KO

PDPK1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 17086-1-AP

Synonymes

hPDK1, PDK1, PDPK1, PRO0461

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Galerie de données de validation

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  WB analysis of MCF-7 using 17086-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17086-1-AP (PDPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 17086-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 17086-1-AP (PDPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17086-1-AP (PDPK1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17086-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17086-1-AP (PDPK1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: MCF-7 cells, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17086-1-AP cible PDPK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDPK1 Protéine recombinante Ag9213
• Nom complet: 3-phosphoinositide dependent protein kinase-1
• Masse moléculaire calculée: 556 aa, 63 kDa
• Poids moléculaire observé: 60-63 kDa
• Numéro d’acquisition GenBank: BC012103
• Symbole du gène: PDPK1
• Identification du gène (NCBI): 5170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

Publications

Species	Application	Title
mouse	WB	Mol Cell; A Non-canonical PDK1-RSK Signal Diminishes Pro-caspase-8-Mediated Necroptosis Blockade.; Authors - Zhang-Hua Yang ; KO Validated
human	WB	PLoS Biol; Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.; Authors - Xuxiao He
human	WB	Free Radic Biol Med; Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis.; Authors - Fang-Yi Su
human	IHC	Front Immunol; Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer; Authors - Yanling Zhou ; KD Validated
mouse	WB	J Innate Immun; Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.; Authors - Yunzhi Liu
human	WB	Biol Trace Elem Res; HIF-1α Mediates Arsenic-Induced Metabolic Reprogramming in Lung Bronchial Epithelial Cells.; Authors - Wenjuan Wang