Anticorps Polyclonal de lapin anti-Podoplanin

Podoplanin Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 11629-1-AP

Synonymes

AGGRUS, D2 40, D2 40,M2A, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 11629-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11629-1-AP (Podoplanin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11629-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11629-1-AP (Podoplanin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HUVEC using 11629-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug Podoplanin antibody (11629-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu placentaire humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

11629-1-AP cible Podoplanin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Podoplanin Protéine recombinante Ag2201
• Nom complet: podoplanin
• Masse moléculaire calculée: 17 kDa, 25 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC022812
• Symbole du gène: PDPN
• Identification du gène (NCBI): 10630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podoplanin was identified as a 43 kDa glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

Publications

Species	Application	Title
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
human	IHC	Elife; Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.; Authors - Marko Z Nikolić
human	WB,IHC	PLoS Pathog; Kaposi's sarcoma-associated herpesvirus promotes mesenchymal-to-endothelial transition by resolving the bivalent chromatin of PROX1 gene.; Authors - Yao Ding
human	WB,IF	PLoS Pathog; Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis; Authors - Weikang Chen
human	IHC	J Infect Dis; Platelets direct monocyte differentiation into epithelioid-like multinucleated giant foam cells with suppressive capacity upon mycobacterial stimulation.; Authors - Yonghong Feng
human	IHC	Int J Mol Sci; Increased Chymase-Positive Mast Cells in High-Grade Mucoepidermoid Carcinoma of the Parotid Gland; Authors - Hiromi Nishimura

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amelia (Verified Customer) (07-22-2022)

PDPN was used for mass cytometry, conjugated in-house.

• Applications: Other