Anticorps Recombinant de lapin anti-PDRG1

• Phare
• Validé par KD/KO

PDRG1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250203E9

N° de cat : 85851-4-PBS

Synonymes

C20orf126, p53 and DNA damage regulated 1, p53 and DNA damage-regulated protein 1, PDRG

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Galerie de données de validation

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  WB analysis using 85851-4-RR (same clone as 85851-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85851-4-RR (PDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85851-4-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 85851-4-RR (same clone as 85851-4-PBS)

  WB result of PDRG1 antibody (85851-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDRG1 transfected Jurkat cells. This data was developed using the same antibody clone with 85851-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85851-4-RR (same clone as 85851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85851-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85851-4-RR (same clone as 85851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85851-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85851-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85851-4-RR against Human PDRG1 were performed. The affinity constant is 0.653 nM.

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Informations sur le produit

85851-4-PBS cible PDRG1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDRG1 Protéine recombinante Ag9109
• Nom complet: p53 and DNA damage regulated 1
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 15-16 kDa
• Numéro d’acquisition GenBank: BC009334
• Symbole du gène: PDRG1
• Identification du gène (NCBI): 81572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.

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