Anticorps Recombinant de lapin anti-PDRG1

• Phare
• Validé par KD/KO

PDRG1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

250203E9

N° de cat : 85851-4-RR

Synonymes

C20orf126, p53 and DNA damage regulated 1, p53 and DNA damage-regulated protein 1, PDRG

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Galerie de données de validation

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  WB analysis using 85851-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85851-4-RR (PDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 85851-4-RR

  WB result of PDRG1 antibody (85851-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDRG1 transfected Jurkat cells.

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  IHC staining of mouse cerebellum using 85851-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 85851-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85851-4-RR (PDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85851-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85851-4-RR against Human PDRG1 were performed. The affinity constant is 0.653 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules Jurkat, cellules MCF-7, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85851-4-RR cible PDRG1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDRG1 Protéine recombinante Ag9109
• Nom complet: p53 and DNA damage regulated 1
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 15-16 kDa
• Numéro d’acquisition GenBank: BC009334
• Symbole du gène: PDRG1
• Identification du gène (NCBI): 81572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.

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