Anticorps Polyclonal de lapin anti-PDS5A

• Phare
• Validé par KD/KO

PDS5A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17485-1-PBS

Synonymes

SCC112, SCC 112, PIG54, PDS5, KIAA0648

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Galerie de données de validation

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  WB analysis using 17485-1-AP (same clone as 17485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 17485-1-AP (same clone as 17485-1-PBS)

  WB result of PDS5A antibody (17485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HEK-293 cells. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 17485-1-AP (same clone as 17485-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 17485-1-AP (same clone as 17485-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 17485-1-AP (same clone as 17485-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17485-1-AP (same clone as 17485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17485-1-AP (PDS5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 17485-1-AP (same clone as 17485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (17485-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17485-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17485-1-PBS cible PDS5A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDS5A Protéine recombinante Ag11525
• Nom complet: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• Masse moléculaire calculée: 1297 aa, 147 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC009650
• Symbole du gène: PDS5A
• Identification du gène (NCBI): 23244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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