Anticorps Monoclonal anti-PDS5A

• Phare
• Validé par KD/KO

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8D4

N° de cat : 67520-1-PBS

Synonymes

1C8D4, Cell proliferation-inducing gene 54 protein, KIAA0648, PDS5, PIG54

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Galerie de données de validation

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  WB analysis using 67520-1-Ig (same clone as 67520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67520-1-Ig (PDS5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67520-1-Ig (same clone as 67520-1-PBS)

  WB result of PDS5A antibody (67520-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HeLa cells. This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67520-1-Ig (same clone as 67520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67520-1-Ig (same clone as 67520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67520-1-Ig (same clone as 67520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67520-1-Ig (same clone as 67520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67520-1-PBS cible PDS5A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDS5A Protéine recombinante Ag11525
• Nom complet: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• Masse moléculaire calculée: 1297 aa, 147 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC009650
• Symbole du gène: PDS5A
• Identification du gène (NCBI): 23244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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