Anticorps Recombinant de lapin anti-PDS5B

PDS5B Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250461B12

N° de cat : 86088-2-RR

Synonymes

Androgen-induced proliferation inhibitor, Androgen-induced prostate proliferative shutoff-associated protein AS3, APRIN, AS3, CG008

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Galerie de données de validation

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  WB analysis using 86088-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86088-2-RR (PDS5B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86088-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDS5B antibody (86088-2-RR, Clone: 250461B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 86088-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5B antibody (86088-2-RR, Clone: 250461B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86088-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86088-2-RR against Human PDS5B were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules SKOV-3
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86088-2-RR cible PDS5B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDS5B Protéine recombinante Ag27977
• Nom complet: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• Masse moléculaire calculée: 165 aa
• Poids moléculaire observé: 166 kDa
• Numéro d’acquisition GenBank: NM_015032
• Symbole du gène: PDS5B
• Identification du gène (NCBI): 23047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDS5B (PDS5 Cohesin Scc1-like Protein B) is a protein associated with sister chromatid cohesion and maintenance of chromosome structure. It plays a significant role in cell cycle regulation, DNA repair, and gene expression. PDS5B interacts with the cohesin complex to ensure the proper separation of sister chromatids during cell division. Additionally, PDS5B is crucial in DNA damage repair, particularly during homologous recombination, helping to maintain genomic stability. It also indirectly affects gene expression and chromatin structure by modulating the function of the cohesin complex. PDS5B is widely expressed during embryonic development and is highly expressed in the brain and testes in adulthood. Its dysfunction is associated with multiple diseases, including Cohesinopathies and Cornelia de Lange syndrome.

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