Anticorps Recombinant de lapin anti-PEA15

• Phare
• Validé par KD/KO

PEA15 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241044G8

N° de cat : 83958-2-PBS

Synonymes

241044G8, Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1

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Galerie de données de validation

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  WB analysis using 83958-2-RR (same clone as 83958-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83958-2-RR (PEA15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 83958-2-RR (same clone as 83958-2-PBS)

  WB result of PEA15 antibody (83958-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEA15 transfected COLO 320 cells. This data was developed using the same antibody clone with 83958-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00916-1

  Cytometric bead array standard curve of MP00916-1, PEA15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83958-2-PBS. Detection antibody: 83958-1-PBS. Standard: Ag13782. Range: 3.125-200 ng/mL.

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  Affinity and Kinetic Characterization of 83958-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83958-2-RR against Human PEA15 were performed. The affinity constant is 0.306 nM.

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Informations sur le produit

83958-2-PBS cible PEA15 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PEA15 Protéine recombinante Ag13782
• Nom complet: phosphoprotein enriched in astrocytes 15
• Masse moléculaire calculée: 130 aa, 15 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: BC002426
• Symbole du gène: PEA15
• Identification du gène (NCBI): 8682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.

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