Anticorps Recombinant de lapin anti-PEA15

• Phare
• Validé par KD/KO

PEA15 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241044G8

N° de cat : 83958-2-RR

Synonymes

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

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Galerie de données de validation

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  WB analysis using 83958-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83958-2-RR (PEA15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 83958-2-RR

  WB result of PEA15 antibody (83958-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEA15 transfected COLO 320 cells.

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  Affinity and Kinetic Characterization of 83958-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83958-2-RR against Human PEA15 were performed. The affinity constant is 0.306 nM.

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Applications testées

• Résultats positifs en WB: cellules C6, cellules COLO 320, cellules MG U-87, cellules Neuro-2a, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83958-2-RR cible PEA15 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PEA15 Protéine recombinante Ag13782
• Nom complet: phosphoprotein enriched in astrocytes 15
• Masse moléculaire calculée: 130 aa, 15 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: BC002426
• Symbole du gène: PEA15
• Identification du gène (NCBI): 8682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.

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