Anticorps Polyclonal de lapin anti-PEBP1

• Phare
• Validé par KD/KO

PEBP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10575-1-AP

Synonymes

RKIP, HCNP, HCNPpp, Neuropolypeptide h3, PBP

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Galerie de données de validation

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  WB analysis of mouse brain using 10575-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10575-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10575-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10575-1-AP (RKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10575-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10575-1-AP (RKIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

10575-1-AP cible PEBP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEBP1 Protéine recombinante Ag0864
• Nom complet: phosphatidylethanolamine binding protein 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC008714
• Symbole du gène: PEBP1
• Identification du gène (NCBI): 5037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

Publications

Species	Application	Title
human	IHC	FASEB J; Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair.; Authors - Jemma Evans
human	IHC	Anticancer Res; Differential Protein Expression of Lymph Node Metastases of Papillary Thyroid Carcinoma Harboring the BRAF Mutation.; Authors - Park Won Seo WS
mouse	WB	J Ethnopharmacol; AR/RKIP pathway mediates the inhibitory effects of icariin on renal fibrosis and endothelial-to-mesenchymal transition in type 2 diabetic nephropathy; Authors - Wenhui Yao ; KD Validated
human	IHC	Medicine (Baltimore); Prognostic significance of RKIP, TGM2, and CMTM4 expression in oral squamous cell carcinoma; Authors - Tianyu Luo

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