Anticorps Recombinant de lapin anti-PEG3

PEG3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241234C5

N° de cat : 84030-5-RR

Synonymes

241234C5, KIAA0287, paternally expressed 3, Paternally-expressed gene 3 protein, PW1

---

Galerie de données de validation

• 

  WB analysis of TT using 84030-5-RR

  TT cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 84030-5-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 84030-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84030-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 84030-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PEG3 antibody (84030-5-RR, Clone: 241234C5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 84030-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PEG3 Recombinant antibody (84030-5-RR, Clone:241234C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 84030-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84030-5-RR against Human PEG3 were performed. The affinity constant is 52.3 pM.

---

Applications testées

• Résultats positifs en WB: TT cells, cellules HepG2
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84030-5-RR cible PEG3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PEG3 Protéine recombinante Ag20132
• Nom complet: paternally expressed 3
• Masse moléculaire calculée: 1588 aa, 181 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: BC150272
• Symbole du gène: PEG3
• Identification du gène (NCBI): 5178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

{{ptg:RelatedPrimaryAntibodies}}