Anticorps Polyclonal de lapin anti-PELO

• Phare
• Validé par KD/KO

PELO Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10582-1-PBS

Synonymes

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

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Galerie de données de validation

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  WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

  IP result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody (10582-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10582-1-PBS cible PELO dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PELO Protéine recombinante Ag0873
• Nom complet: pelota homolog (Drosophila)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC005889
• Symbole du gène: PELO
• Identification du gène (NCBI): 53918
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

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