Anticorps Recombinant de lapin anti-PELO

PELO Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243137F4

N° de cat : 85743-1-PBS

Synonymes

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

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Galerie de données de validation

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  WB analysis using 85743-1-RR (same clone as 85743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85743-1-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85743-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85743-1-RR (same clone as 85743-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PELO antibody (85743-1-RR, Clone: 243137F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85743-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02111-1

  Cytometric bead array standard curve of MP02111-1, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-1-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85743-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85743-1-RR against Human PELO were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85743-1-PBS cible PELO dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PELO Protéine recombinante Ag0873
• Nom complet: pelota homolog (Drosophila)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC005889
• Symbole du gène: PELO
• Identification du gène (NCBI): 53918
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

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