Anticorps Polyclonal de lapin anti-PELP1

PELP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30135-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30135-1-AP (same clone as 30135-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30135-1-AP (PELP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 30135-1-AP (same clone as 30135-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 30135-1-AP (same clone as 30135-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PELP1 antibody (30135-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30135-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30135-1-PBS cible PELP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PELP1 Protéine recombinante Ag32720
• Nom complet: proline, glutamate and leucine rich protein 1
• Masse moléculaire calculée: 1130 aa, 120 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC069058
• Symbole du gène: PELP1
• Identification du gène (NCBI): 27043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

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