Anticorps Monoclonal anti-PELP1

PELP1 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B11E4

N° de cat : 67050-1-PBS

Synonymes

1B11E4, HMX3, MNAR, Modulator of non-genomic activity of estrogen receptor, Proline-, glutamic acid- and leucine-rich protein 1

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Galerie de données de validation

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  WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67050-1-Ig (same clone as 67050-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67050-1-Ig (same clone as 67050-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67050-1-PBS cible PELP1 dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PELP1 Protéine recombinante Ag25729
• Nom complet: proline, glutamate and leucine rich protein 1
• Masse moléculaire calculée: 1130 aa, 120 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC069058
• Symbole du gène: PELP1
• Identification du gène (NCBI): 27043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

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