Anticorps Recombinant de lapin anti-PEMT

PEMT Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242150D5

N° de cat : 85099-1-PBS

Synonymes

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85099-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

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Informations sur le produit

85099-1-PBS cible PEMT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PEMT Protéine recombinante Ag8034
• Nom complet: phosphatidylethanolamine N-methyltransferase
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC050593
• Symbole du gène: PEMT
• Identification du gène (NCBI): 10400
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

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