Anticorps Polyclonal de lapin anti-PER1

PER1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(20)

Avis (1)

N° de cat : 13463-1-AP

Synonymes

hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)

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Galerie de données de validation

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  WB analysis of A549 using 13463-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13463-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13463-1-AP

  Immunofluorescent analysis of Hela cells, using PER1 antibody 13463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cérébral humain, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

13463-1-AP cible PER1 dans les applications de WB, IHC, IF/ICC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PER1 Protéine recombinante Ag4249
• Nom complet: period homolog 1 (Drosophila)
• Masse moléculaire calculée: 1290 aa, 136 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC028207
• Symbole du gène: PER1
• Identification du gène (NCBI): 5187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .

Publications

Species	Application	Title
		Cell Metab; Dysfunctional circadian clock accelerates cancer metastasis by intestinal microbiota triggering accumulation of myeloid-derived suppressor cells; Authors - Jing-Lin Liu
mouse	WB	Nat Commun; Angptl8 mediates food-driven resetting of hepatic circadian clock in mice.; Authors - Siyu Chen
human	IF	Theranostics; Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.; Authors - Mark A Naven
human	IHC	J Transl Med; Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy; Authors - Xiaohui Yang
human	WB,ChIP	Biochim Biophys Acta Mol Basis Dis; Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome; Authors - Yuanyuan Chen
mouse	WB	Int J Mol Sci; Uncovering the Novel Role of NR1D1 in Regulating BNIP3-Mediated Mitophagy in Ulcerative Colitis; Authors - Yidong Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-05-2023)

The antibody works well. I have been using this antibody for human cardiomyocytes, human keratinocytes cell lines, and mouse tissues such as the liver and skin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

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