Anticorps Monoclonal anti-PER2 (isoform 1)

PER2 (isoform 1) Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(14)

CloneNo.

2E6C3

Avis (1)

N° de cat : 67513-1-Ig

Synonymes

PER2, PER 2, hPER2, FASPS, 2E6C3

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Galerie de données de validation

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  WB analysis using 67513-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67513-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67513-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules BxPC-3, cellules HL-60, cellules K-562, cellules L02, cellules THP-1, cellules Y79
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

67513-1-Ig cible PER2 (isoform 1) dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PER2 (isoform 1) Protéine recombinante Ag29489
• Nom complet: period homolog 2 (Drosophila)
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 150~160 kDa
• Numéro d’acquisition GenBank: NM_022817
• Symbole du gène: PER2
• Identification du gène (NCBI): 8864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

Publications

Species	Application	Title
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
human	WB	CNS Neurosci Ther; Cellular iron depletion enhances behavioral rhythm by limiting brain Per1 expression in mice; Authors - Qiong Wu
human	WB	J Biol Chem; M54 selectively stabilizes the circadian clock component of CRY1 and enhances the period of circadian rhythm at cellular level; Authors - Zeynep Melis Gül
human	WB	Cancer Manag Res; Circadian Clock Protein PERIOD2 Suppresses the PI3K/Akt Pathway and Promotes Cisplatin Sensitivity in Ovarian Cancer.; Authors - Zhaoxia Wang
human	WB	J Immunol Res; Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.; Authors - Xiyan Tan
human	WB,IHC	Front Mol Biosci; Period2 is associated with immune cell infiltration and is a potential diagnostic and prognostic marker for hepatocellular carcinoma; Authors - Xiaolin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-05-2023)

I have been using this antibody for human keratinocytes, human cardiomyocytes and mouse tissues like liver and skin

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

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