Anticorps Monoclonal anti-PER2 (isoform 1)

PER2 (isoform 1) Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E6C3

N° de cat : 67513-1-PBS

Synonymes

PER2, PER 2, hPER2, FASPS, 2E6C3

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Galerie de données de validation

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  WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

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  WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67513-1-Ig (same clone as 67513-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67513-1-PBS cible PER2 (isoform 1) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PER2 (isoform 1) Protéine recombinante Ag29489
• Nom complet: period homolog 2 (Drosophila)
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 150~160 kDa
• Numéro d’acquisition GenBank: NM_022817
• Symbole du gène: PER2
• Identification du gène (NCBI): 8864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

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