Anticorps Monoclonal anti-PERK/EIF2AK3

PERK/EIF2AK3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3G3F2

N° de cat : 68482-1-Ig

Synonymes

EIF2AK3, PERK, 3G3F2, EC:2.7.11.1, EIF2AK 3

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Galerie de données de validation

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  WB analysis using 68482-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68482-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68482-1-Ig (PERK/EIF2AK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HEK-293, cellules HSC-T6, cellules HuH-7, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

68482-1-Ig cible PERK/EIF2AK3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PERK/EIF2AK3 Protéine recombinante Ag18254
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 3
• Masse moléculaire calculée: 1116 aa, 125 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC126354
• Symbole du gène: PERK
• Identification du gène (NCBI): 9451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Publications

Species	Application	Title
pig	WB	Int J Mol Sci; Evaluation of Cross-Talk and Alleviate Potential of Cytotoxic Factors Induced by Deoxynivalenol in IPEC-J2 Cells Interference with Curcumin; Authors - Qiyuan Wang
human	WB	Cancer Gene Ther; N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress; Authors - Xinyu Sun
human	WB	FASEB J; Luteolin Modulate Endoplasmic Reticulum Stress by Targeting SIRT1 to Ameliorate DSS-Induced Ulcerative Colitis in Mice; Authors - Hai-Xiang Guo
human	WB	Free Radic Biol Med; Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound Healing; Authors - Zucheng Luo

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