Anticorps Recombinant de lapin anti-PERM1

PERM1 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241613B6

N° de cat : 84425-5-PBS

Synonymes

C1orf170, PERM, 241613B6

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Galerie de données de validation

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  IHC staining of mouse skeletal muscle using 84425-5-RR (same clone as 84425-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84425-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84425-5-RR (same clone as 84425-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84425-5-RR (PERM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84425-5-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 84425-5-RR (same clone as 84425-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using PERM1 antibody (84425-5-RR, Clone: 241613B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84425-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84425-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84425-5-RR against Human PERM1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84425-5-PBS cible PERM1 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PERM1 Protéine recombinante Ag23833
• Nom complet: chromosome 1 open reading frame 170
• Numéro d’acquisition GenBank: BC006300
• Symbole du gène: C1orf170
• Identification du gène (NCBI): 84808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PERM1 (PGC-1/ERR-induced regulator in muscle 1) is a muscle-specific protein induced by PGC-1α and ERRα. PERM1 protein expression is mainly restricted to heart and skeletal muscle, indicating PERM1 functions as a tissue-specific regulator of mitochondrial biogenesis in response to exercise (PMID: 33549681, 36028747). Ablation of Perm1 in mice results in reduced protein expression of lipin-1 accompanied by accumulation of specific phospholipid species (PMID: 33549681). There are two major isoforms of Perm1 protein, the short isoform is about 85 kDa, and the long isoform is 105 kDa.

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